منابع مشابه
Gene stacking in plant cell using recombinases for gene integration and nucleases for marker gene deletion
BACKGROUND Practical approaches for multigene transformation and gene stacking are extremely important for engineering complex traits and adding new traits in transgenic crops. Trait deployment by gene stacking would greatly simplify downstream plant breeding and trait introgression into cultivars. Gene stacking into pre-determined genomic sites depends on mechanisms of targeted DNA integration...
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Xenotransplantation from pigs could alleviate the shortage of human tissues and organs for transplantation. Means have been identified to overcome hyperacute rejection and acute vascular rejection mechanisms mounted by the recipient. The challenge is to combine multiple genetic modifications to enable normal animal breeding and meet the demand for transplants. We used two methods to colocate xe...
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Site-specific recombinases are valuable tools for myriad basic research and genome engineering applications. In particular, hybrid recombinases consisting of catalytic domains from the resolvase/invertase family of serine recombinases fused to Cys2-His2 zinc-finger or TAL effector DNA-binding domains are capable of introducing targeted modifications into mammalian cells. Due to their inherent m...
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Understanding how cells are assembled in three dimensions to generate an organ, or a whole organism, is a pivotal question in developmental biology. Similarly, it is critical to understand how adult stem cells integrate into an existing organ during regeneration or in response to injury. Key to discovering the answers to these questions is being able to study the various behaviors of distinct c...
متن کاملDETECTING FAINT GALAXIES BY STACKING AT 24μm
We stack Spitzer 24μm images for ∼ 7000 galaxies with 0.1 ≤ z < 1 in the Chandra Deep Field South to probe the thermal dust emission in low-luminosity galaxies over this redshift range. Through stacking, we can detect mean 24μm fluxes that are more than an order of magnitude below the individual detection limit. We find that the correlations for low and moderate luminosity galaxies between the ...
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ژورنال
عنوان ژورنال: Plant Biotechnology Journal
سال: 2015
ISSN: 1467-7644
DOI: 10.1111/pbi.12459